anti ephrinb3  (R&D Systems)

Journal: Acta neuropathologica

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

doi: 10.1007/s00401-019-02011-1

Figure Lengend Snippet: Fig. 6 EphrinB3 improves SC migration on FN-coated surfaces. a, b Exit of SC entrapped in an agarose drop and seeded on FN + Fc (a) and FN + EphrinB3 (b), scale bar 200 µm. (c) More SC exit the agarose drop 5 h post-seeding on FN + EphrinB3 than on con- trol surface (two-way ANOVA with repeated measures: p = 0.03, F(1,4) = 10.24). d SC migrate over longer distances from the drop-edge 4 h post- seeding (two-way ANOVA with repeated measures: p = 0.02, F(1,4) = 12.74) on FN + EphrinB3-coated surfaces (n = 3 per group). Graphs represent the values of separate experiments (mean ± SD). e Velocity of single SC was measured in different condi- tions. Only SC seeded on FN + EphrinB3 show a sig- nificant increase of migration speed. This increase is reverted when SC are pre-incubated with anti-EphA4 (one-way ANOVA p = 0.002, F(5,93) = 4.1; two-tailed Mann–Whitney test p = 0.002 between control FN + Fc and FN + EphrinB3 group, n = 12–18 per group). In e, n represents tracked single cells of three different experi- ments repeated independently. Data are expressed as mean values ± SD. Single cells were tracked from three different independent experiments, **p < 0.01

Article Snippet: Neutralization of EphrinB3 epitopes in myelin extracts EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology, ratio: 1:1) for 2 h at room temperature prior to the addition to the cells [55].

Techniques: Migration, Incubation, Two Tailed Test, MANN-WHITNEY, Control

Journal: Acta neuropathologica

Article Title: Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling.

doi: 10.1007/s00401-019-02011-1

Figure Lengend Snippet: Fig. 8 Model of the mechanism of guidance and migration of SC after CNS demyelination. a, b SC encountering CNS white matter are activated by the myelin-associated EphrinB3 through EphB6- and EphA4-SC receptors. c, d The activation of these receptors by phospho- rylation impairs SC adhesion to white matter and increases SC expression of Integrinβ1, promoting their adhesion to BV extracellular matrix. e Lesions of white matter undergo the formation and/or remodeling of BV which increases expression of ECM adhesion molecules, such as FN, and further facili- tate SC mobilization towards the lesion

Article Snippet: Neutralization of EphrinB3 epitopes in myelin extracts EphrinB3 epitopes in myelin extract proteins were neutralized by incubation with anti-EphrinB3 antibodies (AF395, R&D system and sc-271328, Santa Cruz Biotechnology, ratio: 1:1) for 2 h at room temperature prior to the addition to the cells [55].

Techniques: Migration, Activation Assay, Expressing